Facts About flow cytometers Revealed
Facts About flow cytometers Revealed
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Facts-enriched characterization of mobile populations. Morphological information and facts from photos can add to your richness of flow cytometry info. As an example, the figure reveals an or else standard apoptosis assay working with Annexin V and PI, including mobile imaging to characterize cells in Every single population to expose morphologically unique options. These insights couldn't have already been gained from multiplex staining by itself.
Regardless of whether you’re a rookie or an expert, investigate our assets to advance your analysis and experience in flow cytometry.
(B) RAW 264.seven macrophages tend to be annotated inaccurately with regard to particle depend because of their complexity and noncircular borders. The volume of singlets accessible for Assessment boosts after the person trains and employs a more precise design.
Learn new insights into your experimental info by visualizing populations using combined fluorescence and morphology information. Our software interface wizards empower downsampling, concatenation (merged data files) and dimensionality tools that happen to be constructed in the computer software for an intuitive, streamlined facts analysis experience.
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In addition, it is actually illuminating to discover the amount of doublets slip by means of classic singlet gates, and good to verify the position of sudden double-positive occasions in my Investigation."
Pair your Attune flow cytometer with an autosampler for greater workflow performance, and insert robotic integration for maximum throughput.
Regular picture good quality even at superior throughput Acoustic concentrating as well as a superior-velocity digital camera combine to image these T cells continuously at very low or higher flow premiums. Effortlessly adjust aim and camera options to meet experimental necessities.
The Attune NxT cytometer website is definitely the reputable workhorse instrument your lab can count on for regular and reliable functionality. The Attune CytPix model incorporates a significant-speed brightfield digital camera and AI-driven impression Assessment computer software, with modern upgrades like equipment Studying applications and an intuitive user interface, enabling Improved facts precision, accuracy, and quicker insights into sample biology.
Possibly most of all, these gatherings incorporate cells of Evidently distinct phenotypes which could lead to incorrect conclusions relating to double optimistic occasions (especially in unusual populations).
Sound waves allow precise cell alignment, resulting in Fantastic info good quality and ~10X more rapidly sample flow rates.
We Beforehand demonstrated the power of imaging Automobile-T/Ramos mobile interactions. Permit’s examine just the populace of best curiosity, the double positive activities, To find out more. We will now use prolonged morphological parameters (right here, circularity and skewness of depth) to even more take a look at the characteristics of these populations and refine gating on these situations, growing knowledge robustness.
Leverage the power of Sophisticated technology and innovation to accelerate your discoveries. Belief in the Attune Flow Cytometer family to deliver the reliability, velocity, and precision that set your work apart.
Flexible payment prepare from Agilent Money Remedies permit you to obtain the important instrumentation you may need for speedy project, or to improve your lab.
At the center of every Attune Flow Cytometer lies our innovative acoustic concentrating technology, which utilizes audio waves to meticulously align cells inside the flow cell. This revolutionary solution surpasses standard hydrodynamic techniques, resulting in more rapidly processing speeds, Outstanding data good quality, and elevated sample throughput.
Because the mobile line had Beforehand been analyzed to the Attune CytPix Flow Cytometer, the researchers went back again to the photographs and were in a position to document the microbial an infection at least 5 times previously.
Agilent Worth Assure assures you at the least ten years of instrument use in the day you purchase. Normally, We're going to credit you Along with the residual worth of the system towards an upgraded model.
What gave the impression to be a homogeneous inhabitants is in fact extra numerous—an insight that should be thought of when interpreting benefits.